WebJan 1, 2014 · Prepare two independent reactions, one for RNA sense and another one for control RNA antisense. A standard labeling reaction with 1 μg linear template DNA. The approximate yield is 10 μg of full-length biotin-labeled RNA per 1 μg of DNA. 13. The quantity of biotinylated RNA should be optimized for each experiment. 14. WebMay 15, 1989 · An alternative nonradioactive labeling method and a highly sensitive technique for detecting specific DNA sequences are described. The labeling method …
Transcriptome-wide discovery of coding and noncoding RNA-binding ... - PNAS
WebPCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I; Primer Extension: Primer Extension with Klenow 3'-5' exo-; 3'-End … WebJan 5, 2024 · Biotinylated RNA pull-down assay. Biotin-labeled hsa_circ_0003258 and oligonucleotide probes (RiboBio Co. Ltd. Guangzhou, China) were mixed with streptavidin magnetic beads (Beaver, China) in RIP buffer for 4 h. Subsequently, the DU145 cell lysate was incubated with the probes complex for 12 h at 4 °C. chinese brown gravy recipe for egg foo yung
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WebJun 24, 2024 · A bioorthogonal chemical approach was also used to study PPIs. The Benjamin M. Swarts group developed a novel bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne with DA as a photocrosslinking group and a biotin affinity handle for pull-down, named BCN-DAz-Biotin (Fig. 29). The BCN-DAz-Biotin probe contains photocrosslinker … WebCircRNA pull-down assay. A biotin-labeled circPTPRA probe was designed and synthesized by RiboBio Co., Ltd. The RNA pull-down assay was performed according to the instruction of the Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Scientific). The probe sequences used for the RNA pull-down assay are summarized in Additional … WebWe constructed a set of biotin-labeled DNA probes to intron 2 RNA and used them to isolate cross-linked chromatin fragments carrying the intronic RNA sequence, according to previously published protocols (39, 40) (Materials and Methods). We showed that these probes specifically pull down intron 2 RNA, but not a nonspecific control (Fig. 5I). grand city property wikipedia