WebpLZ1 4712 bp 600 1200 1800 2400 3000 3600 4200 T7 promoter; not functional for T7 RNA polymerase null strain; IPTG inducible promoter lac operator and LacUV5; compatible with T7 RNA polymerase null strain LacZ rrnB T1 terminator rrnB_T2 terminator fd terminator S-Tag T7 terminator RSF origin; copy number above 100 per cell AmpR promoter RSF ori … Webp15A origin and chloramphenicol resistance (Search Vector Database) Backbone manufacturer Terrence Lai (this study) Backbone size w/o insert (bp) 2024 Total vector size (bp) 2137 Modifications to backbone Modifications between XbaI and PstI from original pSB1C3 plasmid. CAT gene - C435G (nucleotide) - silent mutagenesis to remove BsmBI site
Origin of replication of Escherichia coli plasmid RSF 1030.
WebSpeed is less than 5000×g. 5.Store the plasmid at -20 ℃. pRSF-1b vector Sequence Copy Sequence Download GeneBank File (.gb) LOCUS Exported 3669 bp ds-DNA circular SYN 20-1-2014 DEFINITION Bacterial vector with an RSF 1030 origin for the regulated expression of N-terminally 6xHis-tagged proteins. ACCESSION . VERSION . WebSep 1, 2015 · RSF1030 origin plasmids are compatible with standard cloning plasmids. The RSF1030 origin plasmids are available in both medium and high copy versions. A series … bonefish check gift card balance
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WebFeb 1, 2006 · We analyzed the structural and functional organization of the ColE2 origin by using various derivatives carrying deletions and single-base-pair substitutions. The origin may be divided into three subregions: subregion I, which is important for stable binding of the Rep protein; subregion II, which is important for binding of the Rep protein… WebBacterial vector with an RSF 1030 origin for the co-expression of two genes. Part ID 720JU2V; Parent -Name pRSFDuet-1; Version -Division -Template - Collection -Part Status Editable check_circle; Public check_circle; Certified check_circle; Length … bonefish christmas cosmo