Web保存条件：-20℃保存，至少两年有效。 注意事项： 碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向[email protected]索取。 sgRNA与质粒及其序列信息，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何 … WebpUltra was cloned by Yildirim Dogan ( [email protected] ). It is described in the associated article (PMID 22427958) and also in PMID 24630793. This is a 3rd generation Lentiviral vector with an internal Ubiquitinc Promoter. By cloning into the compatible cloning sites* (XbaI and BamHI) downstream of EGFP-P2A, you get a bi-cistronic expression ...

Addgene: pWPT-GFP

Web碧云天生产的DH5α超级感受态细胞，英文名DH5α Super Competent Cells，是一种可以用于常规质粒高效转化的质粒克隆用E. coli DH5α即用型化学感受态细胞。使用pUC19质粒进行热激活转化，转化效率可以高达1×109 cfu/μg DNA以上。 WebStbl3 Growth instructions Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C Copy number High Copy Gene/Insert Gene/Insert name EGFP Insert Size (bp) 716 Cloning Information Cloning method Restriction Enzyme 5′ cloning site See map (not destroyed) 3′ cloning site See map (not destroyed) 5′ sequencing primer See map fingerhut portable washer

Plasmids 101: Common Lab E. coli Strains - Addgene

WebDam methylase also plays a role because the oriC has 11 5'-GATC-3' sequences (in E. coli). Immediately after DNA replication, the oriC is hemimethylated and sequestered for a period of time. Only after this, the oriC is released and must be fully methylated by Dam methylase before DnaA binding occurs. Role in regulation of protein expression[edit] WebTrans Stbl3 Chemically Competent Cells (Stbl3) are specifically designed for chemical transformation of DNA containing long terminal repeats (LTR) found in lentiviral vectors. … Web270 rows · Patterns of CpG methylation are heritable, tissue specific and correlate with gene expression. Consequently, CpG methylation has been postulated to play a role in … ervin youngblood